푸른숲이 알려드리는 옻나무추출물의 항암효과
옻은 어혈을 풀어주고 피를 맑게 하며, 몸을 보하고 염증을 제거하는 효과가 있다고 알려져 이미 수천년전부터 쓰여져온 약재입니다. 동의보감에서는 옻은, '어혈을 풀고 염증을 제거하며 부인성 질환의 치료와, 피로 회복에 효과가 있다.'고 나타나 있으며, 본초강목에서는 '만성위장병 치료에 효과가 있고 어혈을 제거하며 혈액순환 개선 및 만성질환, 부인병, 중풍 치료에 효과가 있다"고 설명하고 있습니다.
Flavonol-rich RVHxR from Rhus verniciflua Stokes and its major compound fisetin inhibits inflammation-related cytokines and angiogenic factor in rheumatoid arthritic fibroblast-like synovial cells and in vivo models
Lee Jae-Dong, Huh Jeong-Eun, Jeon GeumSeon, Yang Ha-Ru, Woo Hyun-Su, Choi Do-Young, Park Dong-Suk
Rheumatoid arthritis (RA) is an aggressive inflammatory disease in which cytokines/chemokines are thought to recruit leukocytes and induce angiogenesis. The aim of this study is to investigate the effect of flavonol-rich residual layer of hexane fraction from Rhus verniciflua Stokes (RVHxR) and its major compound fisetin on inflammatory cytokine/chemokine production and angiogenic factor in IL-1β-stimulated RA fibroblast-like synovial cells (FLS) and inflammatory in vivo models. Flavonol-rich RVHxR and its major compound fisetin significantly inhibited IL-1β-induced FLS proliferation in a dose-dependent manner. Flavonol-rich RVHxR and fisetin significantly decreased IL-1β-induced inflammatory cytokines (TNF-α, interleukin (IL)-6)/chemokines (IL-8, monocyte chemoattractant protein (MCP)-1), and vascular endothelial growth factor (VEGF) of RA FLS. Flavonol-rich RVHxR dose dependently diminished the phophorylation of extracellular signal regulated kinase (ERK) and phospho-Jun NH(2)-terminal kinase (JNK), and its down regulation induced by RVHxR at nontoxic concentrations, while activated the phosphorylation of p38 MAPK in IL-1β-stimulated RA FLS. The p38 specific inhibitor SB203580 cotreatment with RVHxR effectively increased the expression of VEGF and blocked the phosphorylation of p38 MAPK in IL-1β-stimulated RA FLS, confirming a critical role of p38 MAPK pathway in angiogenesis inhibition. In experimental inflammation-related models, flavonol-rich RVHxR and fisetin have shown significant anti-inflammatory activities on vascular permeability, leukocyte migration and cellular immunity. Also, flavonol-rich RVHxR and fisetin treatments significantly reduced the incidence and severity of collagen-induced arthritis model. These results suggest that RVHxR and its major compound fisetin have shown potent suppressive effects on some inflammatory cytokines/chemokines and angiogenic factor in IL-1β-stimulated RA FLS and inflammatory in vivo models. We believe that flavonol-rich RVHxR is a potential therapeutic agent in the treatment of inflammatory and angiogenesis related diseases.
옻나무 추출물의 피세틴(fisetin)은 염증과 혈관신생(angiogenesis)으로 인한 질환에 유효할 것으로 보인다.
Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua
Park Kun-Young, Jung Geun-Ok, Lee Kyung-Tae, Choi Jongwon, Choi Moo-Young, Kim Gab-Tae, Jung Hyun-Ju, Park Hee-Juhn
Pretreatment of the methanolic extract of the heartwood of Rhus verniciflua (Anacardiaceae) to rats prevented the activation of hepatic microsomal cytochrome P450enzymes, inhibition of hepatic glutathione S-transferase by bromobenzene treatment, respectively, and therefore significantly decreased malondialdehyde content in the rat. The Ames test showed that the addition of 1.0 mg/plate of the methanolic extract or the EtOAc fraction of the Rhus verniciflua heartwood extract potentially inhibited the mutagenicity by aflatoxin B1. Column chromatography of the EtOAc fraction yielded four flavonoids, garbanzol (1), sulfuretin (2), fisetin (3), fustin (4), mollisacasidin (5). When these components were subjected to the Ames test, it was found that sulfuretin might effectively prevent the metabolic activation or scavenge electrophilic intermediates capable of causing mutation. In contrast, fustin showed a dose-independent antimutagenic activity and it has mutagenic/antimutagenic activity. However, a mixture of sulfuretin and fustin (1:1) exhibited dose-dependent antimutagenicity indicating that sulfuretin inhibited the mutagenicity of fustin. These results suggest that the extract of Rhus verniciflua heartwood containing flavonoid complex could be a potent anticarcinogen.
옻나무 추출물의 플라보노이드 중 설퍼레틴(sulfuretin)과 푸스틴(fustin)을 혼합했을 시 용량에 비례하는 항돌연변이 효과가 있는 것으로 나타났다.
Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells.
Hyon-Seok Jang, Sung-Ho Kook, Young-Ok Son, Jong-Ghee Kim, Young-Mi Jeon, Yong-Suk Jang, Ki-Choon Choi, Ju Kim, Seong-Kyu Han, Kyung-Yeol Lee, Byung-Keon Park, Nam-Pyo Cho, Jeong-Chae Lee
Many studies have suggested that dietary flavonoids are anticancer agents that induce the apoptosis of cancer cells. However, the effects of flavonoids on the induction of apoptosis in osteosarcoma cells are unclear. Previously, a flavonoid fraction, consisting mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, herein named RCMF (the RVS chloroform-methanol fraction), was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS). This study evaluated the effects of RCMF on the proliferation and apoptosis using human osteosarcoma (HOS) cells. The mechanism of growth inhibition of the HOS cells by the flavonoid fraction, RCMF, was also assessed. The results demonstrated that RCMF exhibited sensitive growth inhibition and induced apoptosis in HOS cells. PARP cleavage was closely associated with the RCMF-induced apoptosis of the HOS cells. Furthermore, the activation of caspase 8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are believed to be involved in the RCMF-mediated apoptosis. Collectively, these findings suggest that RCMF is an agent which may be capable of inducing sensitive growth inhibition and apoptosis in HOS cells.
옻나무 추출물의 플라보노이드(flavonoids)들은 사람의 골육종 세포에서 증식 억제와 세포자멸사를 유도하는 효능을 보였다.